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After which, the cells were washed with PBS and followed by fixing using 4% paraformaldehyde for 10 min, permeabilised with 0.5% Triton X-100 for 30 min, blocking with 1% bovine serum albumin for another 30 min

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Cigar use in South Dakota Cigar use in South Dakota is on the declinea promising trend

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In Canada, a wide variety of cigarettes are available, including both local and international brands

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The digested samples were then passed through 40 m strainers, rinsed with PBS, and resuspended in 4 mL of 40% Percoll (SigmaAldrich)

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