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Effects of coffee on 7 nAChR To explore the hypothesis that there might be 7 nAChR agonists in the rich mixture of compounds consumed as coffee, we applied pH-adjusted dark roasted coffee (DRC) made up with the saline components of our Ringers solution (see section 2.5) to oocytes expressing human 7 nAChR without or with the 7 positive allosteric modulator (PAM) PNU-120596 (Hurst et al., 2005) (Figure 1)