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Purified gDNA was quantified by Qubit BR and 100 ng of gDNA was tagmented to an average length of 600 bp with a Tn5 transposome, purified from pTXB1-Tn5 (Addgene, 60240), assembled with oligos containing a 10-nt unique molecular identifier and then purified using solid-phase reverse immobilization (SPRI) guanidine magnetic beads
We showed that in mice genetically engineered to lack secretory IgA, moDCs activate T lymphocytes white blood cells that fight off viruses and can destroy cells in the process and those T lymphocytes in turn damage the lungs
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He never bough more than 2 ounces of anything, and never took tobacco with him anywhere, he just bought a bag (I mean almost daily) and smoked some and put the remainder in a large jar
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