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A total of 2 mL of saliva was collected from each participant and stored in labeled sterile cryovials with the addition of Halt Protease and Phosphatase Inhibitor cocktail (1% v/v, ThermoScientific, Waltham, MA) and stored at 80 C for downstream untargeted high-resolution HPLCMS metabolic analysis as previously described 33,34,35,36