Briefly, the qPCR assay was performed with a T100 Thermal Cycler (Bio-Red, United States) in 20 L reaction mixtures, which contained 1 L of templates, 10 L of the Taq Plus Master Mix (2), 0.8 L of each primer, and 7.4 L of distilled water
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[198] Michael Musto, a journalist for W magazine, wrote that in her film roles, Garland "could project decency, vulnerability and spunk like no other star and she wrapped it up with a tremulously beautiful vocal delivery that could melt even the most hardened troll." [197] By the time of her death in 1969, Garland had appeared in more than 35 films