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In animals used for cFos experiments, trunk blood was collected from cardiac puncture following pentobarbital (150 mg/kg, i.p.) injection before perfusion

The film blends multiple genresaction, drama, satireand its momentum largely hinges on the tension between iconic characters: the duo Django and Schultz versus the local authorities and the social order of the plantation

To select a CpG position for the mouse control gene, we selected a human CpG position which was not differentially methylated in newborn cord blood (human genome GRCh38/hg38, PRKAA1 cg13345558, position chr5:40796738), as reported in the PACE study