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Elevated levels of multiple cytokines including CCL2, IL-4, IL-13, IL-10, M-CSF, and TNF- were found in the lungs of ENDS-exposed mice ( Figures 2CH )

Excess body weight can restrict chest expansion and impair respiratory mechanics, making it harder to clear the airways

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Although our cloning procedure did not, against expectations, lead exclusively to lines with homogenous GFP expression, clearly the cloning method is an effective way to substantially increase the number of homogenous lines