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Circulation: Cardiovascular Quality and Outcomes , January 2017

RNA extraction, quantification of miRNA expression and qPCR Total RNA was extracted from the lung tissue samples of the screening cohort and from PBECs derived from non-COPD and COPD patients by TRIzol reagent method (Molecular Research Center, Cincinnati, OH) according to manufacturer's guidelines

The new study wasn't designed to find out if people who vape or smoke tobacco have a higher chance of catching COVID-19 in the first place, and the jury is still out on that, the researchers noted

However, shisha is served to people aged below 21 only up till 10:00 PM