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While age, gender, and urban versus rural residence also shape these disparities, there remains a pressing need for longitudinal studies to track smoking behaviors and oral health outcomes over time, for culturally tailored interventions targeting high-risk populations, and for research exploring the intersection of oral health disparities and tobacco use across diverse populations and settings

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Finally, the optimized data were processed using a sequence denoising method (DADA2/Deblur) to acquire the representative amplicon sequence variants (ASVs) and their abundance information