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Protein concentrations were determined using a BCA protein assay (Thermo Fisher Scientific) and 60 g of protein/lane were separated by SDSPAGE using e-PAGEL (ATTO Co., Tokyo, Japan) and were electro transferred onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories Inc., Hercules, CA) for 30 min at 25 V
A reduced glutathione supplement isn't just a hopeful ideaits a scientifically-backed tool to help fortify your body's defenses, strengthen your immunity, and reclaim your vitality from the inside out
57 Jimenez-BlascoD.Busquets-GarciaA.Hebert-ChatelainE.SerratR.Vicente-GutierrezC.IoannidouC.et al (2020)