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The cDNA was then subjected to quantitative real-time PCR (10 ng per reaction) carried out with TaqMan Universal PCR Master Mix (Life Technologies, Carlsbad, CA, USA) using the 7300 Real-Time PCR System (Life Technologies) with pre-developed TaqMan probe/primer combinations for UCP-1 (Mm00494069_m1) and glyceraldehyde-3-phosphate dehydrogenase (Mm99999915_g1) optimized by the manufacturer

Discovery of a Fluorogenic Probe for In Situ Pyruvate Kinase M2 Isoform (PKM2) Labeling through Chemoselective S(N)Ar with a Binding Site Lysine Residue

Clinically, G6PD deficiency has been linked to increased risk of Hepatitis A and B infections (99, 100), which may be related to deficient interferon responses in hepatocytes due to insufficient ROS generation by NADPH oxidase, as has been described for Dengue virus infection in G6PD-deficient monocytes (101)

Mounjaro, the top-selling drug by value in India, has sold over 600 crore since its March 2025 launch, a record for any category of drug ever